Acetyl Tripeptide-59 Amide: What Is It, Cosmetic Uses, Benefits & Side Effects

Curious about this ingredient? In this article we're explaining exactly what it is and why it's used within cosmetic formulations.
Updated on: June 23, 2025
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All information on this page is verified using The Personal Care Products Council's (PCPC) INCI database. Our ingredient analyses are based exclusively on PCPC's technical data to ensure accuracy and reliability.

What Is Acetyl Tripeptide-59 Amide?

Acetyl Tripeptide-59 Amide is a lab crafted peptide created by attaching an acetyl group to Tripeptide-59, a short chain of three amino acids. The acetyl tip helps protect the peptide and makes it easier for skin to accept. First explored by ingredient houses looking for gentle anti-aging options, it gained popularity in the late 2010s once in-vitro tests showed it could improve skin feel and softness.

Manufacturing starts with fermenting plant-sourced or synthetic amino acids, linking them in a precise order to build Tripeptide-59, then adding the acetyl group and converting the end of the chain to an amide. This controlled process keeps the molecule small and uniform so it can sit comfortably on skin without breaking down too quickly.

You will most often spot Acetyl Tripeptide-59 Amide in silky serums, lightweight moisturizers, sheet masks and targeted eye or neck creams aimed at smoothing texture or boosting suppleness. Because it is water soluble it slips easily into gels, lotions or emulsions without affecting scent or color.

Acetyl Tripeptide-59 Amide’s Cosmetic Benefits/Uses

In skincare formulas this peptide plays one main role that can elevate the overall experience and performance of a product.

As a skin conditioning agent Acetyl Tripeptide-59 Amide helps skin feel softer, more elastic and well hydrated. By supporting the surface matrix it can leave a smoother finish, making fine lines look less pronounced and giving a comfortable after-feel that encourages regular use of the product.

Who Can Use Acetyl Tripeptide-59 Amide

This peptide is generally well tolerated across all skin types. Its lightweight water based nature means oily or combination skin will not feel weighed down while dry or mature skin can enjoy the added softness and suppleness. Sensitive skin usually finds it non irritating because the molecule is small and free of fragrance or harsh solvents. At standard use levels it is not known to clog pores so acne prone users can typically apply it without worsening breakouts.

Acetyl Tripeptide-59 Amide is produced from plant sourced or fully synthetic amino acids, not animal tissue, so it is suitable for vegans and vegetarians as long as the finished product is certified cruelty free by the brand.

Current research shows no specific issues for women who are pregnant or breastfeeding when using this ingredient topically. Still, this is not medical advice and anyone expecting or nursing should run any skincare product past their healthcare provider to be safe.

The peptide does not increase photosensitivity and can be used morning or night. A daily broad spectrum sunscreen is still recommended to protect the skin improvements you gain.

Potential Side Effects/Adverse Reactions

Responses to topical Acetyl Tripeptide-59 Amide differ from person to person. The following are potential side effects that remain uncommon when the ingredient is formulated and used as directed.

  • Mild redness or warmth for a few minutes after application
  • Transient itching or tingling especially on very reactive skin
  • Localized dryness or flaking if combined with strongly exfoliating products
  • Contact dermatitis in individuals allergic to peptides or other formula components

If any irritation persists or worsens stop use immediately and consult a qualified healthcare professional.

Comedogenic Rating

Rating: 0 out of 5

Acetyl Tripeptide-59 Amide is a very small water soluble molecule that does not form an oily film or harden inside pores, so it earns the lowest score on the comedogenic scale. It stays in the water phase of a formula and leaves no residue that could mingle with sebum.

This makes it suitable for skin that is prone to acne or frequent breakouts.

If congestion occurs after using a product with this peptide the culprit is likely another heavier ingredient rather than the peptide itself.

Summary

Acetyl Tripeptide-59 Amide is a lab crafted three-amino-acid peptide that conditions skin by boosting softness, helping it hold moisture and smoothing the surface so fine lines look less obvious.

Though not as well known as bigger name peptides it is slowly popping up in modern serums, sheet masks and eye creams thanks to its light feel and easy compatibility with other actives.

Current research shows it carries a low risk of irritation, is non comedogenic and is suitable for vegan routines, but skin can be unpredictable so it is wise to patch test any new product that features this peptide before adding it to a daily lineup.

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