Tripeptide-9 Citrulline: What Is It, Cosmetic Uses, Benefits & Side Effects

Curious about this ingredient? In this article we're explaining exactly what it is and why it's used within cosmetic formulations.
Updated on: June 30, 2025
Share:
Inside this article:

We verify all information on this page using publicly available nomenclature standards from The Personal Care Products Council (PCPC), the European Commission's CosIng database and documentation provided directly by ingredient manufacturers. Our analysis is based on technical data from these sources to ensure accuracy and reliability.

What Is Tripeptide-9 Citrulline?

Tripeptide-9 Citrulline is a lab-made peptide created by linking three small amino acids—aspartic acid, lysine and valine—to citrulline, an amino acid naturally found in the body. This marriage of building blocks produces a short, skin-friendly chain that can signal and support healthy looking skin. Peptide technology gained traction in the late 1990s as brands searched for gentle yet effective anti-aging solutions, and Tripeptide-9 Citrulline soon found its way into skin care formulas aiming to boost firmness without heaviness.

The ingredient is produced through solid-phase peptide synthesis, a precise method that strings the amino acids together one by one. After the tripeptide is built, chemists attach citrulline in a controlled reaction, then purify the blend so it is safe for topical use.

You will most often spot Tripeptide-9 Citrulline in lightweight serums, firming moisturizers, eye creams, sheet masks, after-sun gels and other leave-on treatments that promise smoother, more elastic skin.

Tripeptide-9 Citrulline’s Cosmetic Benefits/Uses

In skin care, Tripeptide-9 Citrulline serves one main purpose that delivers several visible perks.

Skin conditioning: The peptide helps skin hold on to water, which keeps the surface soft and plump. At the same time it can support the skin’s own collagen network, giving a firmer feel and reducing the look of fine lines. Because it is lightweight, it absorbs quickly and layers well under other products, leaving skin silky rather than greasy.

Who Can Use Tripeptide-9 Citrulline

Tripeptide-9 Citrulline is gentle enough for nearly every skin type. Dry skin benefits from its water-binding talent, oily and combination skin appreciate its weightless feel and sensitive skin usually tolerates it because it lacks fragrance and harsh solvents. Those with an existing allergy to peptides or any other ingredient in the finished formula should avoid it, otherwise it is broadly suitable.

The peptide is produced entirely in the lab without animal-derived starting materials so it aligns with vegan and vegetarian preferences.

Current data shows no known risks for pregnant or breastfeeding women when the ingredient is used in topical skin care at standard levels. This is not medical advice and anyone who is pregnant or nursing should confirm the safety of every product with a trusted healthcare provider.

Tripeptide-9 Citrulline does not make skin more sensitive to sunlight so it does not increase the need for extra sun protection beyond a daily broad-spectrum sunscreen that should already be in place. It also pairs well with most other actives such as niacinamide, hyaluronic acid and non-acidic antioxidants.

Potential Side Effects/Adverse Reactions

Side effects from topical Tripeptide-9 Citrulline vary from person to person. The points below list potential reactions yet most people will not experience them when the ingredient is used correctly in a well-formulated product.

  • Temporary tingling or mild warmth immediately after application
  • Redness or irritation on very sensitive skin
  • Allergic contact dermatitis in individuals sensitive to peptides or other formula components
  • Rare acne-like breakouts if the overall product is too rich for clog-prone skin
  • Increased irritation when layered with strong acids or retinoids that compromise the skin barrier

If any negative reaction occurs stop using the product and consult a dermatologist or healthcare professional.

Comedogenic Rating

Rating: 0 / 5 — Tripeptide-9 Citrulline is a small water-soluble peptide that does not leave an oily residue or form a film that might trap dead cells in pores. It is used at low concentrations and readily absorbs, so it poses virtually no risk of clogging follicles.

This makes the ingredient generally suitable for people prone to acne or breakouts.

Since the finished formula determines how pore-friendly a product is, keep an eye on heavier oils or waxes that may be included alongside the peptide.

Summary

Tripeptide-9 Citrulline is a lab-crafted peptide that conditions skin by binding water and encouraging a stronger collagen network which helps improve hydration, firmness and overall suppleness. It is lightweight fast absorbing and compatible with most other actives.

The peptide enjoys moderate popularity, showing up mainly in mid-range and premium serums moisturizers and eye creams rather than mass-market lines.

Current data points to an excellent safety profile with low irritation and no photosensitivity. Still everyone’s skin is unique so it is wise to patch test any new product containing Tripeptide-9 Citrulline before full use.

Was this article helpful?
More from Glooshi:
ADVERTISEMENT
Get all our top headlines in beauty.
Delivered right to your inbox each week. Zero spam, all goodness, opt-out at anytime.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Get the latest beauty news, top product recommendations & brand-exclusive discount codes direct to your inbox.
Send good feedback:

All feedback is greatly appreciated, anonymous, and will be used to improve the quality of our articles.

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Send bad feedback:

All feedback is greatly appreciated, anonymous, and will be used to improve the quality of our articles.

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Search