Acetyl Tripeptide-54 Amide: What Is It, Cosmetic Uses, Benefits & Side Effects

Curious about this ingredient? In this article we're explaining exactly what it is and why it's used within cosmetic formulations.
Updated on: June 23, 2025
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All information on this page is verified using publicly available nomenclature standards and reference materials from The Personal Care Products Council (PCPC) and the European Commission's CosIng database. Our analyses are based on technical data from these sources to ensure accuracy and reliability.

What Is Acetyl Tripeptide-54 Amide?

Acetyl Tripeptide-54 Amide is a lab-crafted peptide made by adding an acetyl group to a short chain of three amino acids. The end of that chain is turned into an amide form which helps keep it stable and skin friendly. Peptides first drew attention in the late 1990s for their role in cell communication and skin repair. As research grew, chemists began tweaking natural peptide structures to make them more effective and that is how Acetyl Tripeptide-54 Amide found its way into skin care labs.

The ingredient is produced through a controlled peptide synthesis carried out in stainless steel reactors. Each amino acid is linked in sequence, then the chain is acetylated, and finally converted to an amide. After purification and quality checks the finished powder is ready to be blended into cosmetic bases.

You will usually spot Acetyl Tripeptide-54 Amide in products that target firmness and smoothness. Common formats include lightweight serums, sheet masks, eye creams, leave-on moisturizers and high-performance night treatments. Brands favor it because it pairs well with other actives like hyaluronic acid, niacinamide and antioxidants without losing potency.

Acetyl Tripeptide-54 Amide’s Cosmetic Benefits/Uses

This peptide is prized for the way it conditions skin and supports a healthier look.

  • Skin conditioning: Helps improve softness and suppleness, supports an even texture and can make skin feel more resilient over time which enhances the overall feel of a formula and upgrades the user experience

Who Can Use Acetyl Tripeptide-54 Amide

This peptide sits comfortably on most skin types, including dry, normal, oily and combination. Its lightweight nature makes it friendly to sensitive skin as well, as it is free from common irritants like fragrance or dyes. There are no known reasons it would be unsuitable for any particular skin type, though individuals with a history of peptide allergies should proceed with added care.

Because Acetyl Tripeptide-54 Amide is synthesized entirely in a lab without animal-derived raw materials it is considered vegan and vegetarian friendly.

No data suggest that topical use poses a risk to women who are pregnant or breastfeeding. Still this is not medical advice and anyone who is expecting or nursing should clear any new skincare product with a doctor before adding it to a routine.

The ingredient does not make skin more sensitive to sunlight so it is not classed as photosensitizing. Daily sunscreen is still recommended, but there is no special need to increase sun protection solely because of this peptide.

Potential Side Effects/Adverse Reactions

Responses to Acetyl Tripeptide-54 Amide vary from person to person. The points below outline potential side effects that are considered uncommon. When the ingredient is formulated correctly most users enjoy it without issue.

  • Mild redness or warmth
  • Temporary stinging upon application
  • Localized itching
  • Rare allergic contact dermatitis in individuals sensitive to peptides

If any of these effects occur discontinue use and consult a healthcare professional for guidance.

Comedogenic Rating

Rating: 0 / 5 (non-comedogenic)

Acetyl Tripeptide-54 Amide is a small water-soluble molecule that does not leave an oily film on skin or block pores. It is typically used at low concentrations in lightweight formulas, which further reduces any risk of clogging. For these reasons it earns the lowest possible comedogenic score.

Suitable for acne-prone or breakout-prone skin.

Because the peptide is often combined with other ingredients, pore-clogging potential will depend on the full formula not just the peptide itself.

Summary

Acetyl Tripeptide-54 Amide is a lab-engineered peptide that conditions skin helping it feel softer and more resilient. By supporting the skin’s natural repair signals it can subtly improve texture and overall smoothness without heaviness or irritation.

While not yet as famous as peptides like Matrixyl or copper peptides it is steadily showing up in modern serums eye creams and masks favored by brands looking for gentle performance boosters.

Current data point to a high safety profile with only rare reports of mild irritation. Still it is smart to patch test any new product that contains this peptide to confirm personal tolerance.

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