R-Bacillus Licheniformis Keratinase: What Is It, Cosmetic Uses, Benefits & Side Effects

Curious about this ingredient? In this article we're explaining exactly what it is and why it's used within cosmetic formulations.
Updated on: June 30, 2025
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We verify all information on this page using publicly available nomenclature standards from The Personal Care Products Council (PCPC), the European Commission's CosIng database and documentation provided directly by ingredient manufacturers. Our analysis is based on technical data from these sources to ensure accuracy and reliability.

What Is R-Bacillus Licheniformis Keratinase?

R-Bacillus Licheniformis Keratinase is a lab made version of a natural enzyme called keratinase that normally comes from the Bacillus licheniformis bacterium. Scientists copy the gene that makes keratinase, place it in harmless E. coli bacteria, then let those bacteria grow in large tanks. During fermentation the E. coli produce the enzyme, which is later purified so only the single chain protein with its 379 amino acids remains. This controlled process gives a consistent, high-quality ingredient without using animal or plant materials.

The enzyme first drew attention in the textile and cleaning industries because it can break down tough keratin found in wool, hair and nails. Its gentle yet effective action soon made it attractive for beauty products. Today formulators add it to skincare and nail care lines to help soften hard surfaces and improve the feel of skin and nails.

You will most often see r-Bacillus Licheniformis Keratinase in specialty masks, hand and foot treatments, anti-aging creams, cuticle oils, nail strengtheners and exfoliating lotions.

R-Bacillus Licheniformis Keratinase’s Cosmetic Benefits/Uses

This ingredient offers targeted benefits that improve both skin and nail products.

  • Nail Conditioning: Helps soften and smooth the nail plate and surrounding cuticle so nails look healthy and feel less brittle. Its mild keratin-breaking action can reduce buildup on the nail surface allowing other conditioning agents to penetrate better.
  • Skin Conditioning: Gently loosens dead surface cells leading to smoother, brighter skin without harsh scrubbing. This can enhance softness and support a more even texture over time.

Who Can Use R-Bacillus Licheniformis Keratinase

This enzyme suits most skin types including oily, dry, combination and even sensitive skin because it works gently at the surface. Those with an already damaged or highly inflamed skin barrier may want to wait until their skin has calmed since any exfoliating action could add to discomfort.

The ingredient is produced in E. coli through fermentation with no animal derived inputs so it is appropriate for vegans and vegetarians.

No data indicates special risks for pregnant or breastfeeding women when the enzyme is used topically in cosmetic amounts. That said this is not medical advice and anyone who is expecting or nursing should show the full product ingredient list to their healthcare provider just to be safe.

R-Bacillus Licheniformis Keratinase does not make skin more sensitive to sunlight so it is not considered photosensitizing. It also blends well with most common skincare actives which makes it easy to slot into a routine.

Potential Side Effects/Adverse Reactions

Responses to topical R-Bacillus Licheniformis Keratinase differ from person to person. The points below outline potential side effects but most users experience none of them when the product is formulated and used correctly.

  • Mild tingling or warmth right after application
  • Temporary redness or irritation in very sensitive skin
  • Allergic contact dermatitis if someone is specifically sensitized to the protein
  • Excess dryness or flaking if paired with other strong exfoliants and used too often
  • Over-thinning of the nail plate when used in high concentration leave-on nail products for prolonged periods

If any unwanted reaction occurs stop using the product and consult a healthcare professional or dermatologist.

Comedogenic Rating

Rating: 0/5

R-Bacillus Licheniformis Keratinase is a water-soluble protein with no oily or waxy residues so it does not clog pores. It works on the surface to break down keratin without leaving behind occlusive films that could trap sebum or bacteria. For these reasons formulators consider it non-comedogenic.

Suitable for acne-prone and breakout-prone skin types.

Because it is an enzyme rather than an emollient its presence rarely affects the overall pore-clogging potential of a finished product unless that product also contains heavy oils or butters.

Summary

R-Bacillus Licheniformis Keratinase is a lab-generated version of keratinase that conditions skin and nails by gently clipping away excess keratin. On nails it softens the plate and cuticle letting moisturizers penetrate better. On skin it loosens dead cells for a smoother brighter surface.

The enzyme is still a niche addition so you will mainly see it in targeted hand masks foot peels cuticle oils and a few exfoliating creams but its vegan eco-friendly production is helping it gain traction in newer formulations.

Topical use is considered very safe with low risk of irritation or pore blockage. As with any new ingredient it is best to patch test a small area first to check personal tolerance before regular use.

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