Tetrapeptide-64: What Is It, Cosmetic Uses, Benefits & Side Effects

Curious about this ingredient? In this article we're explaining exactly what it is and why it's used within cosmetic formulations.
Updated on: June 30, 2025
Share:
Inside this article:

We verify all information on this page using publicly available nomenclature standards from The Personal Care Products Council (PCPC), the European Commission's CosIng database and documentation provided directly by ingredient manufacturers. Our analysis is based on technical data from these sources to ensure accuracy and reliability.

What Is Tetrapeptide-64?

Tetrapeptide-64 is a lab-made chain of four amino acids: glycine, histidine, proline and valine. Because it is synthetic, manufacturers can create it under controlled conditions for consistent quality rather than relying on animal or plant extraction. The cosmetic industry began exploring short peptides like Tetrapeptide-64 in the early 2000s when research showed these mini proteins could signal the skin to look smoother and better hydrated. Production starts with individual amino acids that are linked together through a process called solid-phase peptide synthesis, followed by purification to remove any residual reactants. You will commonly spot Tetrapeptide-64 in anti-aging serums, firming creams, sheet masks, after-sun treatments and recovery-focused moisturizers where formulators want a gentle yet targeted skin revitalizer.

Tetrapeptide-64’s Cosmetic Benefits/Uses

This peptide is included in skincare mainly for one job, but that role can have several visible perks for the skin.

Skin conditioning: Tetrapeptide-64 helps improve the overall feel and appearance of the skin. By supporting a more resilient surface, it can make skin look smoother, more supple and comfortably hydrated, which is why brands often place it in products aimed at reducing signs of fatigue or early aging.

Who Can Use Tetrapeptide-64

Tetrapeptide-64 is generally suitable for all skin types, including dry, oily, combination and sensitive, because it is a lightweight conditioning peptide that does not add heavy oils or fragrances. People with very reactive skin should still check the full ingredient list of a finished product to ensure no added irritants are present.

Because the peptide is made synthetically from individual amino acids it is considered vegan and vegetarian friendly; no animal parts or by-products are needed in its manufacture. Those who avoid animal testing should verify that the finished product has not been tested on animals, as that depends on each brand’s policy and local regulations.

Current data show no specific concerns for topical use during pregnancy or while breastfeeding, yet clinical studies on expectant or nursing mothers are limited. This is not medical advice and anyone who is pregnant or nursing should review any new skincare product with a qualified healthcare provider to be safe.

Tetrapeptide-64 does not increase photosensitivity, so it can be used day or night without making skin more prone to sunburn. Normal daily sunscreen use is still recommended for overall skin health.

Potential Side Effects/Adverse Reactions

Side effects from topical Tetrapeptide-64 vary from person to person. The points below list potential reactions, though they are uncommon when the ingredient is formulated and used correctly.

  • Redness or mild irritation
  • Itching or stinging sensation
  • Allergic contact dermatitis in rare cases
  • Eye irritation if the product migrates into the eyes
  • Breakouts if the overall formula is too rich or occlusive for acne-prone skin

If any negative reaction occurs stop using the product and consult a healthcare professional.

Comedogenic Rating

Comedogenic rating: 0 out of 5. Tetrapeptide-64 is a small, water-soluble molecule with no oily or waxy portions that could block pores, so it does not create the kind of surface film that triggers blackheads or whiteheads. The peptide is usually incorporated at low percentages and rinses cleanly with normal cleansing, which further lowers any clogging risk. Because of this profile, Tetrapeptide-64 is generally a safe choice for people who are prone to acne or breakouts. If the finished formula pairs the peptide with heavy oils or thick butters those carriers, not the peptide itself, would be the more likely pore-clogging culprits.

Summary

Tetrapeptide-64 is mainly valued for its skin-conditioning talent, helping skin feel smoother, more supple and comfortably hydrated by supporting a stronger surface barrier. It delivers these benefits through cell-signaling activity that encourages the skin to maintain a healthy moisture balance and a resilient texture. While not yet a blockbuster household name like hyaluronic acid or retinol, the peptide has gained steady popularity in higher-end serums and recovery creams where brands want a gentle performance ingredient without adding heaviness.

Current evidence shows Tetrapeptide-64 is safe for topical use across all skin types with a very low risk of irritation or comedogenicity. As with any new skincare ingredient it is smart practice to do a small patch test first to make sure your skin agrees with the overall formula.

Was this article helpful?
More from Glooshi:
ADVERTISEMENT
Get all our top headlines in beauty.
Delivered right to your inbox each week. Zero spam, all goodness, opt-out at anytime.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Get the latest beauty news, top product recommendations & brand-exclusive discount codes direct to your inbox.
Send good feedback:

All feedback is greatly appreciated, anonymous, and will be used to improve the quality of our articles.

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Send bad feedback:

All feedback is greatly appreciated, anonymous, and will be used to improve the quality of our articles.

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Search