Tripeptide-16: What Is It, Cosmetic Uses, Benefits & Side Effects

Curious about this ingredient? In this article we're explaining exactly what it is and why it's used within cosmetic formulations.
Updated on: June 30, 2025
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We verify all information on this page using publicly available nomenclature standards from The Personal Care Products Council (PCPC), the European Commission's CosIng database and documentation provided directly by ingredient manufacturers. Our analysis is based on technical data from these sources to ensure accuracy and reliability.

What Is Tripeptide-16?

Tripeptide-16 is a lab made peptide built from three amino acids: glutamine, glycine and proline. Because it is synthetic, it does not come from plants or animals but is created in controlled laboratory settings where each amino acid is linked together in a precise order. Peptide technology gained traction in skin care during the early 2000s when researchers discovered that short chains of amino acids could signal the skin to look and feel healthier. Chemists form Tripeptide-16 through solid phase peptide synthesis, a step by step process that joins the amino acids on a resin bed then purifies the finished chain.

Today you will find Tripeptide-16 in a variety of cosmetic formulas aimed at keeping skin supple and smooth. It is common in leave on treatments such as serums, moisturizing creams, sheet masks and eye creams as well as richer night balms that focus on a firmer appearance. Brands often pair it with hydrating agents and antioxidants to round out an anti aging routine.

Tripeptide-16’s Cosmetic Benefits/Uses

In formulas Tripeptide-16 acts as a skin conditioning agent. This means it helps the surface of the skin feel softer and more hydrated which supports a fresher look over time.

Who Can Use Tripeptide-16

Tripeptide-16 is generally suitable for all skin types including dry, normal, combination and oily skin because it focuses on conditioning rather than exfoliating or deeply resurfacing. Sensitive skin usually tolerates it well since the peptide is non acidic and has a neutral pH. If a person is extremely reactive to new ingredients they may still want to introduce the product slowly, but there is no built in feature of Tripeptide-16 that makes it harsh or stripping.

The ingredient is fully synthetic and does not rely on any animal derived components, so it is considered appropriate for vegans and vegetarians.

No data shows that Tripeptide-16 poses a special risk to pregnant or breastfeeding women when applied topically. That said this is not medical advice and anyone who is pregnant or nursing should have a quick word with a healthcare provider before adding new skin care products, just to be safe.

Tripeptide-16 does not make the skin more sensitive to sunlight and can be used during the day under sunscreen.

Potential Side Effects/Adverse Reactions

Side effects from topical Tripeptide-16 can vary from one person to another. The points below cover potential issues that have been noted in rare cases. Most users will not experience these reactions when the ingredient is included at normal levels in a well-formulated product.

  • Redness or mild irritation
  • Itching or a tingling sensation shortly after application
  • Allergic contact dermatitis in individuals with a specific peptide sensitivity
  • Interaction with other actives causing temporary stinging, especially on compromised skin

If any of these effects occur the best course of action is to stop using the product and seek guidance from a healthcare professional if symptoms persist.

Comedogenic Rating

Rating: 0/5

Tripeptide-16 is water soluble and used in very small amounts, so it does not clog pores or add extra oil to the skin. Because it is a lightweight peptide that stays on the surface to condition, it earns the lowest possible comedogenic score.

This makes the ingredient suitable for people who are prone to acne or breakouts.

No data suggest it worsens blackheads, whiteheads or cystic acne, even when used daily.

Summary

Tripeptide-16 is a lab made trio of amino acids that works as a skin conditioning agent. By sitting on the surface it helps skin feel softer, look smoother and stay comfortably hydrated. Brands often slot it into serums, moisturizers and eye creams to support a fresh, healthy look.

While not the biggest name in skin care, its use has grown steadily as more formulas lean on peptides for gentle age support without irritation.

Current research shows Tripeptide-16 is safe for most skin types with a very low risk of side effects. As with any new product, it is smart to do a quick patch test first to make sure your skin agrees with the full formula.

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